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(A) NO release rates from bare TPU and hydrogel-coated surfaces measured by a Griess-based assay (n = 6). (B) Fluorescence images of platelet adhesion on bare TPU, PDM, PD@Cu, and PDM@Cu surfaces with and without NO donors. (C) Quantification of platelet adhesion by LDH assay (n = 6). (D) SEM images of platelet morphology on bare TPU and hydrogel-coated surfaces. (E) <t>APTT</t> and (F) TT of bare TPU and hydrogel-coated surfaces (n = 3). (G) Digital photographs of thrombus formation on bare TPU and hydrogel-coated surfaces after 5, 10, and 15 min during recalcification assays.
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(A) NO release rates from bare TPU and hydrogel-coated surfaces measured by a Griess-based assay (n = 6). (B) Fluorescence images of platelet adhesion on bare TPU, PDM, PD@Cu, and PDM@Cu surfaces with and without NO donors. (C) Quantification of platelet adhesion by LDH assay (n = 6). (D) SEM images of platelet morphology on bare TPU and hydrogel-coated surfaces. (E) <t>APTT</t> and (F) TT of bare TPU and hydrogel-coated surfaces (n = 3). (G) Digital photographs of thrombus formation on bare TPU and hydrogel-coated surfaces after 5, 10, and 15 min during recalcification assays.
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(A) NO release rates from bare TPU and hydrogel-coated surfaces measured by a Griess-based assay (n = 6). (B) Fluorescence images of platelet adhesion on bare TPU, PDM, PD@Cu, and PDM@Cu surfaces with and without NO donors. (C) Quantification of platelet adhesion by LDH assay (n = 6). (D) SEM images of platelet morphology on bare TPU and hydrogel-coated surfaces. (E) <t>APTT</t> and (F) TT of bare TPU and hydrogel-coated surfaces (n = 3). (G) Digital photographs of thrombus formation on bare TPU and hydrogel-coated surfaces after 5, 10, and 15 min during recalcification assays.
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Dojindo Labs cell counting kit 8 reagent
(A) NO release rates from bare TPU and hydrogel-coated surfaces measured by a Griess-based assay (n = 6). (B) Fluorescence images of platelet adhesion on bare TPU, PDM, PD@Cu, and PDM@Cu surfaces with and without NO donors. (C) Quantification of platelet adhesion by LDH assay (n = 6). (D) SEM images of platelet morphology on bare TPU and hydrogel-coated surfaces. (E) <t>APTT</t> and (F) TT of bare TPU and hydrogel-coated surfaces (n = 3). (G) Digital photographs of thrombus formation on bare TPU and hydrogel-coated surfaces after 5, 10, and 15 min during recalcification assays.
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(A) NO release rates from bare TPU and hydrogel-coated surfaces measured by a Griess-based assay (n = 6). (B) Fluorescence images of platelet adhesion on bare TPU, PDM, PD@Cu, and PDM@Cu surfaces with and without NO donors. (C) Quantification of platelet adhesion by LDH assay (n = 6). (D) SEM images of platelet morphology on bare TPU and hydrogel-coated surfaces. (E) APTT and (F) TT of bare TPU and hydrogel-coated surfaces (n = 3). (G) Digital photographs of thrombus formation on bare TPU and hydrogel-coated surfaces after 5, 10, and 15 min during recalcification assays.

Journal: Bioactive Materials

Article Title: Robust and durable zwitterionic hydrogel coating with catechol-Cu 2+ coordination for antithrombotic and antibacterial blood-contacting devices

doi: 10.1016/j.bioactmat.2026.02.036

Figure Lengend Snippet: (A) NO release rates from bare TPU and hydrogel-coated surfaces measured by a Griess-based assay (n = 6). (B) Fluorescence images of platelet adhesion on bare TPU, PDM, PD@Cu, and PDM@Cu surfaces with and without NO donors. (C) Quantification of platelet adhesion by LDH assay (n = 6). (D) SEM images of platelet morphology on bare TPU and hydrogel-coated surfaces. (E) APTT and (F) TT of bare TPU and hydrogel-coated surfaces (n = 3). (G) Digital photographs of thrombus formation on bare TPU and hydrogel-coated surfaces after 5, 10, and 15 min during recalcification assays.

Article Snippet: Cell counting kit-8 (CCK-8), activated partial thromboplastin time (APTT) kit, thrombin time (TT) test kit, and enhanced BCA protein assay kit were obtained from Beyotime Biotechnology Inc., Shanghai, China.

Techniques: Griess Assay, Fluorescence, Lactate Dehydrogenase Assay